Rotavirus is a viral agent associated with childhood gastroenteritis, being with intestinal parasites, the principal cause of diarrhea in children. In order to detect rotavirus, and intestinal parasites in children from 0 to 5 years old of six indigenous communities in Zulia state, 173 stool samples was recolected, where the presence of rotavirus was determinate using agglutination test. The samples were characterized macroscopically and microscopically by examining with SSF, lugol and concentrate formalin-ether to detect parasitic evolutionary forms. It was determined that the presence of intestinal parasites was 71%, significantly higher than the 16,2% of rotavirus. The most prevalent parasites were Ascaris lumbricoides (32,4%) and Blastocystis spp. (31,2%). Statistically significant relationship between the presence of rotavirus and diarrhea existence (p = 0.0001); more no presence of rotavirus among vaccinated children (p = 0.904 ). About they predominated protozoosis over helminths; as well as polyparasitism (52%). Major parasites associated with rotavirus were Blastocystis spp. and Entamoeba coli, although without statistical significance. It is concluded that the prevalence of rotavirus detected corresponds to that reported in Venezuela for children under 5 years of urban areas

In order to determine the prevalence of intestinal parasites in children from an indigenous community in the municipality Machiques de Perija of Zulia state, they were analyzed fecal samples from 139 children aged between 0-12 years old. Each stool specimen was conducted a coproparasitological fresh examination with physiological saline 0.85% and temporary colorations NAIR and lugol, as well, Ritchie´s concentration technique. An overall prevalence of children parasitized 87.7% was observed, no significant differences in the studies of the variables parasitosis and sex. It was observed a predomine of polyparasitism (68,35%) on monoparasitism (20,15%). As for the age group, stratum 2 to 6 years was the mostly parasitized (43.92%). The x2 revealed that if there is significant relationship between variables and age parasitism. Parasitic species obtained a higher prevalence were: Entamoeba coli (46.06%), Blastocystis sp. (43.16%), Trichuris trichiura (30.94%) and Ascaris lumbricoides (21.58%) throughout the study group. These results are similar to those obtained in previous research. The high prevalence of protozoan suggest a pattern of direct transmission between the study population or through consumption of water and/or fecally contaminated food

Urinary tract infections (UTI) are a common problem in medical practice, the etiology and susceptibility that produce the UTI may vary over time. It was conducted a study of no-experimental descriptive in order to detect the causative organisms of urinary tract infections and antimicrobial susceptibility in older adults residing in a health center of Carabobo state. They were evaluated 57 urine samples for bacteriological culture and the recovered microorganisms underwent susceptibility testing by disk diffusion method in agar (Kirby-Bauer). The most frequently isolated microorganisms in the ITU were Escherichia coli (53.84% and Klebsiella pneumoniae (15.40%), among others. In The antimicrobial susceptibility testing, the antibiotics that showed better antimicrobial activity were nitrofurantoin and amikacin (84.62%) each one, followed by gentamicin (76.92%) for all the Gramnegative bacilli recovered. The greater resistance was observed in ampicillin (61.54%), followed by trimethoprim-sulfamethoxazole (53.85%) and ciprofloxacin (46.15%), in enteric Gram-negative bacilli. In the present study was found a greater presence of Escherichia coli in the UTI as well as better antimicrobial activity of nitrofurantoin, amikacin and gentamicin against all the bacteria recovered

Klebsiella pneumoniae is an important opportunist pathogen that mainly cause outbreaks in health institutions, when it acquires the ability to produce carbapenemase, becomes resistant to betalactamics and other groups of antimicrobials as quinolones and aminoglycosides. The aim of this study was to determine the presence of K. pneumoniae strains producing KPC type carbapenemase in clinical isolates of hospitalized patients from three intensive care units of a health institution. All K. pneumoniae strains isolated from routine cultures performed to patients held in the three units were studied, identification was performed by automated equipment Vitek 2C, susceptibility tests were made by Bauer -Kirby method and detection of KPC carbapenemase was done by the modified Hodge test, imipenem and meropenem MIC and blaKPC gene amplification. The results indicate a high prevalence of KPC carbapenemase in the institution, a marked pattern of multidrug resistance was observed in the KPC carbapenemase producing strains, it is recommended to carry out studies to understand the epidemiology and transmission of this resistance mechanism within the institution

Oxacillin-resistant Staphylococcus aureus (ORSA) has remained a major cause of nosocomial infections worldwide. The antibiotic resistance of isolations was determined and we classify them in multidrug-resistant, extensively drug-resistant or pandrug-resistant. The biological samples of patients from a Maracaibo’s Hospital, during September 2013 to February 2014, were processed according to conventional techniques of bacteriology. Antibiotic resistance was determined by disk diffusion method in agar and the mecA gene was detected by polymerase chain reaction. It was observed a low prevalence of nosocomial ORSA (13.86%). The higher antibiotic resistance was observed against erythromycin (66.07%) and a resistance lower than 25% to aminoglycosides, fluoroquinolones, tetracycline and clindamycin. The isolates showed a very low resistance to trimethoprim/sulfamethoxazole and all isolates were susceptible to rifampicin, linezolid, vancomycin and teicoplanin.The majority of isolates had a MSB phenotype (33.93%), with erythromycin resistance and susceptibility to clindamycin. The ORSA isolates in this study had 25 different antibiotypes and the majority of them were multidrug-resistant (55.36%). There was not both extensively drugresistant and pandrug-resistant isolates and the presence of the mecA gene was demonstrated in all isolates of ORSA

Histoplasmosis is a granulomatous disease, produced by Histoplasma capsulatum. This fungus causes a wide range of clinical manifestations; the most common is the pulmonary form. In some cases, atypical presentations are described. Case report: a 70 year old male patient, from Barinas, Venezuela, who worked in a chicken farm, with diagnosis of histoplasmosis and hemolytic anemia is described. The patient improved with antifungal treatment. Conclusions: in Venezuela, an endemic area for this mycosis, clinicians must have a high suspicion index for this disease, taking into account risk factors, such as age and epidemiology. To our knowledge, this is the third case report in the literature

This work reports experience in probable cases of chikungunya fever at adult emergency in a Maracaibo Hospital, Venezuela. It is describes morbidity and mortality between September to December 2014, as well as analysis and clinic diagnosis in acute and complicated cases in a endemic dengue fever zone